Vinogradsky’s columns are known as a model of a microbial community for experiments in laboratory conditions. The article proposes a system of integrated use of Vinogradsky’s columns in the space of the Earth Science Museum as: a) a visual dynamic “living exhibit”, b) an interactive platform of the Youth Museum, and a mobile cluster for exhibitions and the Science Festival, c) a natural educational and methodological tool, and d) a laboratory naturalistic experimental setup. Making a series of Vinogradsky’s columns from different locations of the initial pedo-soil matrix of various compositions allows for a successful combination of scientific, experimental, educational, and demonstration-interactive tasks in the space of a natural science university museum. In the Earth Science Museum at Moscow State University (Hall 21 – East European Plain), a series of Vinogradsky’s columns has been developed, based initially on the materials of bottom sediments samples of pond reservoirs, selected by a team of novice nature explorers (students of Moscow State University and schoolchildren) as part of the project “Youth Museum” of Moscow State University. Two-year monitoring has allowed to obtain new data on the development of microbial communities and to arrange an original cluster in the hall and to present a series of columns at the All-Russian Science Festival.